A STUDY ON PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF QUERCUS MACRANTHERA SUBSP. SYSPIRENSIS (K. KOCH) MENITSKY BRANCH AND LEAF EXTRACTS

Objective: Oak species are medicinal plants with traditional use around the world. These species, which very rich in tannins, have potential as antibacterial agents terms of polyphenolic compounds content. In this study, and phytochemical content branches leaves Quercus macranthera subsp. syspirensis, is endemic to Turkey, were investigated. Material Method: Plant materials collected from Araç (Kastamonu/Turkey) 2020. Methanol extracts prepared dried powdered leaves. The activity test was evaluated by broth microdilution method a minimal inhibition concentration (MIC) against Staphylococcus aureus ATCC 29213, epidermidis 35984, Enterococcus faecalis 29212, Klebsiella pneumoniae 13883, Pseudomonas aeruginosa 27853, Escherichia coli 25922, Acinetobacter baumannii 19606 Bacillus subtilis 6633. GC-MS analysis performed using an Agilent 6890 gas chromatograph equipped 5973N quadrupole mass spectrometer (Agilent, USA). identified comparing spectrum ratio sample data available NIST 2014 Mass Spectral Library. Result Discussion: As result, it found that branch more effective than leaf both showed highest 6633 strain (48.8 µg/ml, 97.6 respectively). also at varying concentrations on other strains.